Application
Flow Cytometry
Sample
Human Cell (MCF-7)
Specification
MCF-7
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: PBS + 0.2% BSA + 0.1% Sodium azide
Sample buffer: PBS + 0.2% BSA + 0.1% Sodium azide
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Live cells
Other product details
Dilution
1/5
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + 0.2% BSA + 0.1% Sodium azide
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The purple profile shows negative control (mouse IgG) vs green profile ab77219 (CD24). Tested in live and fixed cells, works well in both.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 24 2012