Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: form buffy coat after FICOLL
Sample buffer: PBS/BSA 1%/Azide 0.01%
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
lymphocyte gated
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 1%/Azide 0.01%
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Nov 01 2012