Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid ph6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Other product details
Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
This Ab appears to give identical positivity to ab32090.
It should, as it the same clone...-)
Upper image: ab78237
Lower image: near sequential section using anti CD20 clone L26.
It should, as it the same clone...-)
Upper image: ab78237
Lower image: near sequential section using anti CD20 clone L26.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jul 19 2010