Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (raw 264.7 (macrophage))
Specification
raw 264.7 (macrophage)
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/350
Additional data
Additional Notes
Ab119830 (green) against beta-2 integrin subunit was used to stain RAW264.7 macrophage-like cells, and rhodamine-phalloidin (red) was used as a counterstain. The antibody showed beautiful peripheral staining of beta-2, as expected.
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投稿 Jul 01 2013