Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (PBMC derived macrophage)
Permeabilization
Yes - Triton-X-100
Specification
PBMC derived macrophage
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 3% FBS/PBS
Concentration
5 µg/ml
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Both surface and internal staining correspond to previously described staining with CD163. Antibody was confirmed by WB as well.
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投稿 Jul 15 2016