Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human tonsil tissue)
Antigen retrieval step
Other
Permeabilization
No
Specification
human tonsil tissue
Blocking step
Odyssey Blocking Buffer (PBS) as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
No signal when stained on human tonsil tissue. In provided image, blue is DAPI and red is CD163.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC-P and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 10 2017