Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (fibrosarcoma)
Total protein in input
1000 µg
Specification
fibrosarcoma
Immuno-precipitation step
Protein G
Other product details
Concentration
10 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Name
Non-Abcam antibody was used: Rabbit pAb HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100
Additional data
Additional Notes
For figure:
Lane 1 - control (no antibody was used for the IP)
Lane 2: WM15
Lane 1 - control (no antibody was used for the IP)
Lane 2: WM15
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Helen Gillingham
Verified customer
投稿 Apr 09 2008