Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-Buffer
Sample
Human Tissue sections (Gut)
Specification
Gut
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 2%BSA
Dilution
1/5
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated anti mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Abcam response
We appreciate the valuable data provided in this Abreview. To our knowledge ab23602 has not been tested previously in IHC-P. Regrettably the results have not been successful in this case. We would welcome feedback from other researchers using ab23602 in IHC-P.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 23 2014