Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
casein in PBS- DAKO ready to use sera-free protein block as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: DAKO antibody diluent
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: EnVision+ System- HRP labelled polymer anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Additional data
Abcam response
Thank you for your review. I would recommend increasing the incubation with the primary antibody to overnight at 4C for the strongest staining. Please be advised that our Abpromise guarantees our products to work as stated for 12 months from purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Traci Lyons
Verified customer
投稿 Nov 13 2013