Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
Permeabilization
Yes - H2O2
Specification
Skin (epidermis to SQ fat or muscle)
Blocking step
ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% neutral buffered formalin
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Abcam antibody was used but this form would not le
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
Additional data
Additional Notes
The following dilutions were used: 1:50, 1:100, 1:200, 1:400
Abcam response
Thank you so much for testing this antibody in IHC-P. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application.
We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 07 2018