Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human cells grown on interphase co-culture)
Permeabilization
No
Specification
human cells grown on interphase co-culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Dilution
1/60
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Donkey anti-rabbit-Cy3
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
IHC/Fr image of ab52478 stained THP-1 cells (red). Four cell types (EA.hy926 below A549, THP-1 and HMC-1 above) were cultured on membrane inserts, frozen in Tissue-Tek® and cut into 14 µm sections. Sections were fixed for 5 min in 4% formaldehyde at ambient temperature (RT). Blocking of unspecific reactions was performed with 5% donkey serum in PBS for 30 min at RT. THP-1 were then stained with ab52478 (1:60) for 1 h at RT and secondary anti-rabbit-Cy3 (1:250, red) for another hour at RT. Cytoplasm of EA.hy926 and A549 were counterstained with Evans Blue (blue). Nuclei were labelled with Yopro (green) and the section was mounted with ProLong® Diamond Antifade. The picture shows an enlarged section of the membrane with THP-1 labelled in red.
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投稿 May 24 2016