Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive macrophages
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 1%/Azide 0.01%
Secondary antibody
Secondary antibody
None used
Additional data
Abcam response
Thank you for your valuable feedback.
We have not yet tested this antibody with rat cells, so it is possible that the antibody does not cross-react with this species. We appreciate any further feedback in this regard.
We have not yet tested this antibody with rat cells, so it is possible that the antibody does not cross-react with this species. We appreciate any further feedback in this regard.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 27 2012