Anti-CD11a 抗体 [EP1285Y] - BSA and Azide free (ab227119)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD11a antibody [EP1285Y] - BSA and Azide free
CD11a 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cynomolgus monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
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特記事項
ab227119 is the carrier-free version of ab52895.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1285Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Biotin Anti-CD11a antibody [EP1285Y] (ab200022)
- Anti-CD11a antibody [EP1285Y] - Low endotoxin, Azide free (ab246701)
- Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)
- Alexa Fluor® 488 Anti-CD11a antibody [EP1285Y] (ab307764)
- Alexa Fluor® 594 Anti-CD11a antibody [EP1285Y] (ab315256)
- Anti-CD11a antibody [EP1285Y] (ab52895)
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227119の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
組織特異性
Leukocytes. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメイン
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
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別名
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
画像
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Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).
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CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).
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Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-CD11a antibody clone, EP1285Y, in a different buffer formulation (cat# ab52895).
Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (8)
ab227119 は 8 報の論文で使用されています。
- Gao L et al. Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion. Elife 5: (2016). PubMed: 27763263
- Abramowski P et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014). WB ; Human . PubMed: 25181038
- Takahara M et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013). PubMed: 23583640
- Carrillo-de Sauvage MA et al. CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo. PLoS One 7:e30762 (2012). IHC-Fr ; Cynomolgus monkey . PubMed: 22319587
- Jerke U et al. Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J Biol Chem 286:7070-81 (2011). PubMed: 21193407
- Rains JL & Jain SK Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells. Am J Physiol Endocrinol Metab 301:E298-306 (2011). WB, Blocking ; Human . PubMed: 21540444
- Okuzaki D et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
- Tanimoto K et al. Clinical significance of co-expression of CD21 and LFA-1 in B-cell lymphoma. Int J Hematol 89:497-507 (2009). PubMed: 19360456