Anti-CD10 抗体 [56C6] (ab951)
Key features and details
- Mouse monoclonal [56C6] to CD10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD10 antibody [56C6]
CD10 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [56C6] to CD10 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment corresponding to CD10. Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein
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ポジティブ・コントロール
- IHC-P: MDA-MB-231 human breast cancer cells grown in mouse. Renal cell carcinoma tissue.
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特記事項
We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.
This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, Carrier protein
Renoir Red Diluent -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
56C6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab951の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (3) |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block.
Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult.
It is therefore recommended that a light hematoxylin stain be applied.
Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).
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特記事項 |
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IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-). |
ターゲット情報
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機能
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
配列類似性
Belongs to the peptidase M13 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- Unigene: 307734 Human
- Unigene: 296022 Mouse
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別名
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
画像
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Paraffin embedded human renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.
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ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab951 は 36 報の論文で使用されています。
- Koulis A et al. CD10 and Das1: a biomarker study using immunohistochemistry to subtype gastric intestinal metaplasia. BMC Gastroenterol 22:197 (2022). PubMed: 35448971
- Lackie RE et al. Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-ß deposition in a mouse model of Alzheimer's disease. Acta Neuropathol Commun 8:143 (2020). PubMed: 32825842
- Kobayashi T et al. Retrograde transport of masseter muscle-derived neprilysin to hippocampus. Neurosci Lett 698:180-185 (2019). PubMed: 30639512
- Gill M et al. Placental Syncytiotrophoblast-Derived Extracellular Vesicles Carry Active NEP (Neprilysin) and Are Increased in Preeclampsia. Hypertension 73:1112-1119 (2019). PubMed: 30929513
- Hu T et al. Neuroprotection induced by Navß2-knockdown in APP/PS1 transgenic neurons is associated with NEP regulation. Mol Med Rep 20:2002-2011 (2019). PubMed: 31257483