Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat after ficoll preparation
Sample buffer: PBS/BSA 1%/Azide 0.01%
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
none
Permeabilization
No
Gating Strategy
alive lymphocytes
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 1%/Azide 0.01%
Secondary antibody
Secondary antibody
None used
Additional data
Abcam response
Thank you for your valuable feedback.
We have only tested this antibody with mouse cells, and it may not react with the human protein.
We have only tested this antibody with mouse cells, and it may not react with the human protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 23 2012