Anti-Cyclin K 抗体 (ab85854)
Key features and details
- Rabbit polyclonal to Cyclin K
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cyclin K antibody
Cyclin K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cyclin K -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Elephant -
免疫原
Synthetic peptide corresponding to Human Cyclin K aa 500-600.
Database link: O75909 -
ポジティブ・コントロール
- WB: HEK-293T, MCF7, Jurkat, HeLa, HepG2, NIH3T3, CH27, TCMK-1, BW5147.3 and CT26 cell lysates IP: HEK-293T cell lysate IHC-P: Human lung carcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab85854 was affinity purified using an epitope specific to Cyclin K immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85854の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a role in transcriptional regulation. In vitro, is associated with a kinase activity toward both RNA polymerase II C-terminal domain and CDK2 (CAK). -
組織特異性
Ubiquitously expressed. Highest levels in testis. -
配列類似性
Belongs to the cyclin family. Cyclin C subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 8812 Human
- Entrez Gene: 12454 Mouse
- Entrez Gene: 500715 Rat
- Omim: 603544 Human
- SwissProt: O75909 Human
- SwissProt: O88874 Mouse
- Unigene: 510409 Human
- Unigene: 474441 Mouse
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別名
- CCNK antibody
- CCNK_HUMAN antibody
- CPR 4 antibody
see all
画像
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All lanes : Anti-Cyclin K antibody (ab85854) at 0.04 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Exposure time: 75 secondsDetection: Chemiluminescence
Cells prepared using NETN lysis buffer
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Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human lung carcinoma sections labeling Cyclin K with ab85854 at a dilution of 1/5000 (0.2 µg/mL). Detection: DAB
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Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody ab85854 used for IP at 6 µg per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated Cyclin K, ab85854 was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
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All lanes : Anti-Cyclin K antibody (ab85854) at 0.04 µg/ml
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : CH27 whole cell lysate
Lane 3 : TCMK-1 whole cell lysate
Lane 4 : BW5147.3 whole cell lysate
Lane 5 : CT26 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Exposure time: 30 secondsDetection: Chemiluminescence
Cells prepared using NETN lysis buffer
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab85854 は 2 報の論文で使用されています。
- Chai Q et al. HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity. Cell Rep 36:109514 (2021). PubMed: 34380030
- Isa NF et al. HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation. Vaccines (Basel) 9:N/A (2021). PubMed: 34696162