Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500
Detection
Detection method
ecl+
Bands
Specific: 93 kDa Non-specific: 50, 55, 68, 160 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Mouse embryonic stem cell line in lane 2 that is potentially negative for CCK4, as shown by mass spec.
Positive control
Mouse embryonic stem cell line in lanes 1.
Additional data
Additional Notes
Lane 1 is WT mouse ES cells, lane 2 is a cell line that is potentially negative for CCK4, as shown by mass spec. I think this antibody detects CCK4, but I rated it 3 star, because of the background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 23 2014