Application
Western blot
Sample
Human Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) (DyLight 680), Polyclon
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Detection
Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Additional data
Additional Notes
The blot had to undergo a very high exposure to detect anything around 100 kDA which resulted in very high background
Abcam response
Thank you for your review. We do recommend performing WB at a concentration of 2-4 ug/ml for this antibody, so a dilution of 1/250-1/500 incubated overnight at 4C. This may improve your results. Please be advised that our Abpromise guarantee protects antibodies for 12 months from purchase for tested applications and species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 22 2014