Application
Western blot
Sample
Mouse Tissue lysate - other (Brain)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
200 µg
Specification
Brain
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST+0.5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-Rabbit IgG HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 53 kDa Non-specific: 80 kDa
Positive control
Mouse brain extracts
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 14 2017