Anti-CAP1 抗体 (ab88446)
Key features and details
- Mouse polyclonal to CAP1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CAP1 antibody
CAP1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to CAP1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human CAP1 aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q01518 -
ポジティブ・コントロール
- Human colon membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells Methanol treated human HeLa cells Methanol treated human promonocytic THP-1 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88446の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. -
配列類似性
Belongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10487 Human
- Omim: 601896 Human
- SwissProt: Q01518 Human
- Unigene: 370581 Human
- Unigene: 713078 Human
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別名
- Adenylyl cyclase associated protein 1 antibody
- Adenylyl cyclase-associated protein 1 antibody
- CAP 1 antibody
see all
画像
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Anti-CAP1 antibody (ab88446) at 1 µg/ml + Human colon tissue at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Anti-CAP1 antibody (ab88446) at 1 µg/ml + A-431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
All lanes : Anti-CAP1 antibody (ab88446) at 1 µg/ml
Lane 1 : Transfected 293T cell line
Lane 2 : Non-transfected cell line
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunofluorescence image staining human CAP1 in HeLa cells, using ab88446 at 10µg/ml.
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FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human HeLa cells.
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FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human promonocytic THP-1 cells.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab88446 は 2 報の論文で使用されています。
- Jin ZL et al. AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes. FASEB J 34:11292-11306 (2020). PubMed: 32602619
- Jin ZL et al. CAP1-mediated actin cycling via ADF/cofilin proteins is essential for asymmetric division in mouse oocytes. J Cell Sci 131:N/A (2018). PubMed: 30404832