CAMKIV overexpression 293T lysate (whole cell) (ab94283)

製品の概要

  • 製品名
    CAMKIV overexpression 293T lysate (whole cell)
  • 特記事項

    ab94283 is a 293T cell transfected lysate in which Human CAMKIV has been transiently over-expressed using a pCMV-CAMKIV plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. Tissue specificity: Expressed in epithelial ovarian cancer tissue. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. PTM: Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.

アプリケーション

Our Abpromise guarantee covers the use of ab94283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94283 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-CAMKIV antibody (ab88544) at 1/500 dilution

    Lane 1 : CAMKIV overexpression 293T lysate (whole cell) (ab94283)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94283 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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