Application
Western blot
Sample
Mouse Cell lysate - whole cell (EL4)
Loading amount
10000 µg
Specification
EL4
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Other product details
Concentration
1.5 µg/ml
Incubation time
10 minute(s) · Temperature: 20°C · Diluent: 0.5% milk in TBS, 0.1% tween
Secondary antibody
Name
Non-Abcam antibody was used: ECL anti-rabbit IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Western Lightning chemiluminescent reage
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: ~59 kDa Non-specific: 70, 45 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 04 2012