Anti-CAMKIV 抗体 [EP2564Y] (ab75874)
Key features and details
- Rabbit monoclonal [EP2564Y] to CAMKIV
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-CAMKIV antibody [EP2564Y]
CAMKIV 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2564Y] to CAMKIV -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat whole cell lysate and Human brain lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2564Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75874の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
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特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 52 kDa. |
ターゲット情報
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機能
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. -
組織特異性
Expressed in epithelial ovarian cancer tissue. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
細胞内局在
Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 814 Human
- Entrez Gene: 12326 Mouse
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P08414 Mouse
- SwissProt: P13234 Rat
- Unigene: 591269 Human
see all -
別名
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
画像
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All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab75874 は 1 報の論文で使用されています。
- Yan X et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . PubMed: 27611779