Anti-CACNA1H 抗体 [N55/10] (ab84815)
Key features and details
- Mouse monoclonal [N55/10] to CACNA1H
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CACNA1H antibody [N55/10]
CACNA1H 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N55/10] to CACNA1H -
由来種
Mouse -
特異性
No cross-reactivity against Cav1.3 and Cav3.1
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アプリケーション
適用あり: IHC-P, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Fusion protein corresponding to Human CACNA1H aa 1000-1300.
Database link: O95180 -
ポジティブ・コントロール
- IHC-P: Human hippocampus tissue. IHC-Fr: Frozen human brain tissue.
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特記事項
The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N55/10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000.
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WB |
1/1000. Predicted molecular weight: 259 kDa.
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|
IHC-Fr |
1/1000.
|
特記事項 |
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IHC-P
1/1000. |
WB
1/1000. Predicted molecular weight: 259 kDa. |
IHC-Fr
1/1000. |
ターゲット情報
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機能
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. -
組織特異性
Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific. -
関連疾患
Defects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease. -
配列類似性
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily. -
ドメイン
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
翻訳後修飾
In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8912 Human
- Entrez Gene: 58226 Mouse
- Entrez Gene: 114862 Rat
- Omim: 607904 Human
- SwissProt: O95180 Human
- SwissProt: O88427 Mouse
- SwissProt: Q9EQ60 Rat
- Unigene: 459642 Human
see all -
別名
- Alpha1 3.2 antibody
- Alpha13.2 antibody
- CAC1H_HUMAN antibody
see all
画像
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Immunohistochemical analysis of frozen Human brain tissue sections staining CACNA1H. Samples were fixed with 10% Formalin solution and incubated with primary antibody at 1:1000 dilution for 1 hour at room temperature. A biotinylated goat anti-mouse was used as the secondary antibody. Mayer Hematoxylin was used as a nuclear stain.
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Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus tissue labelling CACNA1H with ab84815 at 1:1000 dilution, followed by secondary antibody Goat anti-mouse (green) at 1:50 for 1 hour at RT.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab84815 は 2 報の論文で使用されています。
- Kürtüncü M et al. CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL). Cephalalgia : (2012). WB ; Human . PubMed: 23111027
- Ohkubo T & Yamazaki J T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells. Int J Oncol 41:267-75 (2012). PubMed: 22469755