Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary hippocampal neurons)
Specification
primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - ice-cold methanol (optional)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%BSA+0.1%Triton in 1xPBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
Additional Notes
This antibody did not work for ICC on mouse primary hippocampal neurons. I tried different protocols, including different antibody dilutions. I also did co-stainings with NeuN to make sure that the staining itself is working. In none of the stainings the CABP antibody gave a signal.
Abcam response
Thank you for your valuable feedback.
We have not tested this antibody in ICC/IF yet, so it is possible that it is not suitable for such an application. We appreciate any other feedback in regards to this.
We have not tested this antibody in ICC/IF yet, so it is possible that it is not suitable for such an application. We appreciate any other feedback in regards to this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 15 2012