Anti-C5a-R 抗体 (ab59390)
Key features and details
- Rabbit polyclonal to C5a-R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-C5a-R antibody
C5a-R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to C5a-R -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human C5a-R. non-phosphopeptide derived from human C5a-R around the phosphorylation site of serine 338.
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ポジティブ・コントロール
- WB: Extracts from HeLa cells treated with PMA (125ng/ml, 30mins).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59390の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 728 Human
- Entrez Gene: 12273 Mouse
- Omim: 113995 Human
- SwissProt: P21730 Human
- SwissProt: P30993 Mouse
- SwissProt: Q3TZ86 Mouse
- Unigene: 2161 Human
- Unigene: 247623 Mouse
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別名
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
see all
画像
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All lanes : Anti-C5a-R antibody (ab59390) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins).
Lane 2 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) plus peptide.
Predicted band size: 39 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted? -
ab59390 staining C5a-R in human lung.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab59390 は 9 報の論文で使用されています。
- Li C et al. Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-κB signaling pathway. Phytomedicine 109:154551 (2023). PubMed: 36610119
- Shibata R et al. Administration of C5a Receptor Antagonist Improves the Efficacy of Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cell Transplantation in the Acute Phase of Spinal Cord Injury. J Neurotrauma 39:667-682 (2022). PubMed: 35196890
- Kim EN et al. CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm. PLoS One 16:e0245361 (2021). PubMed: 34428207
- Asare Y et al. Differential Role for Activating Fc?RIII in Neointima Formation After Arterial Injury and Diet-Induced Chronic Atherosclerosis in Apolipoprotein E-Deficient Mice. Front Physiol 11:673 (2020). PubMed: 32625118
- Liu X et al. Panton-Valentine Leukocidin Colocalizes with Retinal Ganglion and Amacrine Cells and Activates Glial Reactions and Microglial Apoptosis. Sci Rep 8:2953 (2018). PubMed: 29440661
- Zhang XY et al. Anaphylatoxin C5a induces inflammation and reduces insulin sensitivity by activating TLR4/NF-kB/PI3K signaling pathway in 3T3-L1 adipocytes. Biomed Pharmacother 103:955-964 (2018). WB . PubMed: 29710512
- Mödinger Y et al. C5aR1 interacts with TLR2 in osteoblasts and stimulates the osteoclast-inducing chemokine CXCL10. J Cell Mol Med 22:6002-6014 (2018). PubMed: 30247799
- Mueller KAL et al. Myocardial expression of the anaphylatoxin receptor C3aR is associated with cardiac inflammation and prognosis in patients with non-ischaemic heart failure. ESC Heart Fail 5:846-857 (2018). PubMed: 30168657
- Zhang C et al. Antagonist of C5aR Prevents Cardiac Remodeling in Angiotensin II-Induced Hypertension. Am J Hypertens N/A:N/A (2014). Mouse . PubMed: 24419904