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Products:Immunology >> Innate Immunity >> Complement >> Classical Pathway
Overview
Product name
Anti-C1q antibody
C1q 抗体 (12件) 一覧
Description
Rabbit polyclonal to C1q
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse C1q.
(Peptide available as ab91203.)
Positive control
This antibody gave a positive signal in Mouse blood plasma tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Innate Immunity >> Complement >> Classical Pathway
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Western blot - C1q antibody (ab64632)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).
Target
Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Involvement in disease
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Post-translational
modifications
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02745
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C1QA antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
Database links
- Entrez Gene: 12259 Mouse
- Entrez Gene: 298566 Rat
- SwissProt: P98086 Mouse
- SwissProt: P31720 Rat
- Unigene: 439957 Mouse
- Unigene: 105647 Rat
Anti-C1q antibody images:
Western blot - C1q antibody (ab64632)
Anti-C1q antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
The 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor.
C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-C1q antibody (ab64632)
ab64632 has not yet been referenced specifically in any publications.
Publishing research using ab64632? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"