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Western blot - C1q antibody (ab64632)

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Product Name
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C1q antibody
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C1q 抗体 (10件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to C1q
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse C1q. (Peptide available as ab91203.)
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Reacts with
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Ms
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Predicted to react
(species key)
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Rat (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Mouse blood plasma tissue lysate.
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Cellular localization
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Cytoplasmic and Secreted
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker Immunology >> Innate Immunity >> Complement >> Classical Pathway
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Relevance
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C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with the tetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and is composed of three basic subunits termed C1QA, C1QB and C1QC which asociate in the proportion 6 A-B subunits (52, 750 Da) and 3 C-C subunits (47,600 Da), to form whole C1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation.
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C1q antibody (ab64632)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 C1q antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for C1q antibody images, references, products related to ab64632 and other tools.
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C1q antibody images:
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Western blot - C1q antibody (ab64632)
C1q antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
The 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor.
C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to C1q
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C1q antibody - more information
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Customer reviews (feedback) regarding C1q antibody |
Customer FAQs regarding C1q antibody |
Protocols for C1q antibody |
Price and availability of products related to C1q antibody |
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C1q antibody - related products:
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C1q antibody - other tools:
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Contact Abcam with a Technical Enquiry about C1q antibody |
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