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ab91203 |
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Anti-C1q antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
The 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor.
C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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