Application
Western blot
Loading amount
60 cells
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (MII oocytes)
Specification
MII oocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/3000
Detection
Detection method
ECL+
Bands
Specific: 130 kDa
Exposure
3 minute(s) and 0 second(s)
Positive control
only marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
直子 木村
Verified customer
投稿 Oct 02 2013