Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Specification
Colon carcinoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Sheep FITC
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100
Additional data
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our Abtrial testing discount program
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eleni Petsalaki
Verified customer
投稿 Dec 03 2012