Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (colon cancer)
Specification
colon cancer
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-Sheep-cy2
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 07 2012