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Anti-BrdU antibody - Proliferation Marker (ab1893)

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Overview

Product name

Anti-BrdU antibody - Proliferation Marker
BrdU 抗体 (17件) 一覧

Description

Sheep polyclonal to BrdU - Proliferation Marker

Tested applications

IHC-FrFl, IHC-P, IHC-Fr, IP, ICC/IF, ELISA, IHC-FoFrmore details

Immunogen

Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 0.15M PBS, pH 7.5

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

  • Immunoprecipitation - BrdU antibody - Proliferation Marker (ab1893)Immunoprecipitation - BrdU antibody - Proliferation Marker (ab1893) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893)Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Cellular localization

Nuclear

Alternative names

  • Bromodeoxyuridine antibody
  • BUdr antibody

Anti-BrdU antibody - Proliferation Marker images:

  Immunoprecipitation - BrdU antibody - Proliferation Marker (ab1893)

Immunoprecipitation - BrdU antibody - Proliferation Marker (ab1893)

ab1893 used in IP.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893)

ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).

This image is courtesy of an Abreview submitted by Prof Yvona Mazurova

See Abreview

  Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893)

Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893)

ab1893 Immunofluorescence data

Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.

Image courtesy of Dr Dan Webber, CARD institute, KCL, London

References for Anti-BrdU antibody - Proliferation Marker (ab1893)

This product has been referenced in:

  • Di Stefano Vet al. Knockdown of Cyclin-dependent Kinase Inhibitors Induces Cardiomyocyte Re-entry in the Cell Cycle. J Biol Chem 286:8644-54 (2011). ICC/IF.Read more (PubMed: 21209082) »
  • Liu Jet al. Regenerated Luminal Epithelial Cells Are Derived from Preexisting Luminal Epithelial Cells in Adult Mouse Prostate. Mol Endocrinol 25:1849-1857 (2011).Read more (PubMed: 21940754) »

See all 24 publications for this product

Publishing research using ab1893? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"