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Anti-BrdU antibody - Proliferation Marker
BrdU 抗体 (17件) 一覧
Sheep polyclonal to BrdU - Proliferation Marker
IHC-FrFl, IHC-P, IHC-Fr, IP, ICC/IF, ELISA, IHC-FoFrmore details
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 0.15M PBS, pH 7.5
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Protein G purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers
Immunoprecipitation - BrdU antibody - Proliferation Marker (ab1893)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893)
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Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893)
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Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FrFl: Use at an assay dependent concentration.
IHC-P: Use a concentration of 10 µg/ml
IHC-Fr: Use a concentration of 10 µg/ml
IP: Use a concentration of 25 - 100 µg/ml.
ICC/IF: Use at an assay dependent dilution. (PubMed: 21118958)
ELISA: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19332057)
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Nuclear
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody - Proliferation Marker (ab1893)

ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).
This image is courtesy of an Abreview submitted by Prof Yvona Mazurova
Immunocytochemistry/ Immunofluorescence - BrdU antibody - Proliferation Marker (ab1893)

ab1893 Immunofluorescence data
Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.
Image courtesy of Dr Dan Webber, CARD institute, KCL, London
This product has been referenced in:
See all 24 publications for this product
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ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).
This image is courtesy of an Abreview submitted by Prof Yvona Mazurova

ab1893 Immunofluorescence data
Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.
Image courtesy of Dr Dan Webber, CARD institute, KCL, London
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