If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab1893 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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A customer has the following enquiry; |
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ANSWER: |
Thank you for contacting us. |
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Thanks for take a look over this inquiry. attached please find our answers to the questions you asked. I hope it to be of assistance for you the provide us with good and helpful support. I look forward to your reply soon, and a possible replacement for this item expected. Best regard |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (ab1893) with the order number XXXXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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Can you tell me if this product also detects iododeoxyuridine (IDU)? If so, what is the percentage of reactivity? |
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ANSWER: |
Thank you for your enquiry. Unfortunately this antibody has not been tested against IDU. |
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Does the antibody pick up free Brdu? Also, do you know how I can remove unincorporated Brdu...perhaps dialysis? |
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ANSWER: |
Thank you for your enquiry and your interest in our products. The Brdu antibody should recognize both incorporated and free Brdu. The free Brdu should wash away in the experiment. I hope this information will be useful. |
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Dear Sir or Madam, I need to replenish my supply of an antibody raised in sheep against BrdU. The antibody is made by [competitor 1], which is where I last purchased it. However, [competitor 1] no longer sells to end users directly and they directed me to several suppliers, Abcam being one of them. I believe that Abcam is selling the [competitor 1] antibody under catalogue number AB1893 and is charging $299 for 125ug. [competitor 2] also is marketing the [competitor 1] antibody and is charging $305 for 250ug, essentially half the price that Abcam is charging. I have no experience with [competitor 2]. In contrast, my experience with Abcam has been very positive, and therefore I would like to purchase the sheep ant-BrdU antibody from Abcam if you will match [competitor 2]'s price. Thanks very much. Sincerely,
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ANSWER: |
Thank you for your email and your kind words about Abcam. Unfortunately we cannot match our competitor's price. We spend alot of time calculating the amount of antibody we can sell for a particular price based not only on the cost of bringing the antibody to market, whether the antibody is produced by Abcam or outside of Abcam, but also on a number of other factors. For example, if you look at the datasheet for ab1893 you will see that we have published feedback from customers as Abreviews, we have published previous enquiries, and we have published images and references. We have an Abpromise stating that if ab1893 does not perform as described on the datasheet, notify us within 90 days of delivery, so that we can help you or offer you a replacement or a refund. As our founder has stated many times we hope that when customers purchase an antibody from us they realize that they are purchasing more than the antibody in the vial. You are also purchasing the other intangibles that allow you to state "I have no experience with [competitor 2]. In contrast, my experience with Abcam has been very positive, and therefore I would like to purchase the sheep anti-BrdU antibody from Abcam..." I hope you will understand and still consider Abcam as the primary source for your antibodies. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab1893 at a 1/50 dilution staining rat brain tissue sections by IHC-P. The incubation time with the primary antibody was overnight at 4°C. This image shows double-staining by polyclonal sheep BrdU (labeled with Cy3 - red) and nestin 401v (labeled with Alexa Fluor® 488 - green). The detection of proliferative (BrdU-positive) astrocytes (some of them express also nestin, which demonstrates that they are reactive and of lower differentiation compared with later stages of reactive astrocytes) within the rat striatum 6 days after striatal neurotoxic lesion (by quolinic acid).
This image is courtesy of an Abreview submitted by Prof Yvona Mazurova
ab1893 Immunofluorescence data
Tissue culture cells were labelled prior to transplantation and then identified in in vivo tissue using ab1893 sheep polyclonal BrdU antibody (10ug/ml incubated overnight at room temperature) with a TRITC conjugated secondary antibody.
Image courtesy of Dr Dan Webber, CARD institute, KCL, London
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