Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - 0.3% triton x-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3 · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.3% Triton X-100, 3% NGS 1% BSA
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: DyLight 594 Anti-mouse IgG (H+L)
Host species: Horse
Clonality: Monoclonal
Conjugation: DyLight 594
Host species: Horse
Clonality: Monoclonal
Conjugation: DyLight 594
Additional data
Additional Notes
Brdu (red) double staining with anti-neun (green)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jacob Garza
Verified customer
投稿 Sep 14 2017