Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Treatment
Differentiating Conditions (Minus LIF Medium)
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Blotto
Secondary antibody
Name
Non-Abcam antibody was used: Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
50 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 45, 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Utkarsh Kapoor
Verified customer
投稿 Dec 28 2012