Anti-Bovine Papilloma Virus E2 抗体 [1H10] (ab77174)
Key features and details
- Mouse monoclonal [1H10] to Bovine Papilloma Virus E2
- Suitable for: WB, ICC/IF
- Reacts with: Bovine papillomavirus
- Isotype: IgG
製品の概要
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製品名
Anti-Bovine Papilloma Virus E2 antibody [1H10]
Bovine Papilloma Virus E2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1H10] to Bovine Papilloma Virus E2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Bovine papillomavirus -
免疫原
Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli.
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エピトープ
Mapped to amino acids 208-218 (RPDGVWVASEG) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1H10 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77174の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa.
WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
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ICC/IF |
Use a concentration of 1 µg/ml.
1/1000 for 1 hour at RT, in PBS with 1% BSA. |
特記事項 |
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WB
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa. WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
ICC/IF
Use a concentration of 1 µg/ml. 1/1000 for 1 hour at RT, in PBS with 1% BSA. |
ターゲット情報
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関連性
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
細胞内局在
Host cell nucleus -
参照データベース
- SwissProt: P03122 Bovine papillomavirus
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別名
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
画像
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All lanes : Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)
Lane 1 : Recombinant purified Bovine Papillomavirus E2 protein at 10000 µg
Lane 2 : Recombinant purified Bovine Papillomavirus E2 protein at 5000 µg
Lane 3 : Recombinant purified Bovine Papillomavirus E2 protein at 1000 µg
Lane 4 : Recombinant purified Bovine Papillomavirus E2 protein at 500 µg
Predicted band size: 45 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab77174 は 2 報の論文で使用されています。
- Kurg R et al. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. J Virol 80:11218-25 (2006). WB . PubMed: 16943289
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). WB, ELISA . PubMed: 10233926