Application
Immunoprecipitation
Immuno-precipitation step
Other - Pierce Protein A/G Magnetic Beads
Sample
Mouse Cell lysate - whole cell (Heart)
Specification
Heart
Total protein in input
50 µg
Other product details
Incubation time
45 minute(s) · Diluent: Co-IP/Lysis buffer
Western Blot antibody
Name
Non-Abcam antibody was used: Þ-Arrestin 2 (C16D9)
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Western blot was performed after a 2D-cleanup of eluted sample and resuspended in 20 uL industrial strength buffer. The entire sample was loaded into a 12% bis-tris acrylamide gel under denaturing conditions and run for 1 hr 15 min under constant voltage, 200V, in 1X tris-glycine run buffer, 1% SDS, and transferred onto a 0.2 um PVDF membrane for 2 hrs under constant voltage, 25V, in 1X CAPS, pH 11. The membrane was then blocked for 30 min in 5% milk in TBS-T, and probed for beta-arrestin 2, from cell signaling technology, at a concentration of 1:1000 in 1% BSA in TBS-T. Secondary anti-rabbit antibody was used at 1:40,000 in 2.5% Milk.
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投稿 Oct 09 2014