Application
Western blot
Sample
Human Cell lysate - whole cell (Colorectal cancer cell line SW480)
Loading amount
20 µg
Specification
Colorectal cancer cell line SW480
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-goat HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2500
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 46 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 02 2008