Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK-293 cells transfected with human Bestrophin 1)
Permeabilization
Yes - 0.5% TX 100, 5% goat serum, 1XPBS
Specification
HEK-293 cells transfected with human Bestrophin 1
Blocking step
goat serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%goat serum, 0.1%TX100, 1XPBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rabbit Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Bestrophin transfected in HEK-293 cells is labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Feb 15 2008