Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 transfected with human bestrophin 1)
Gel Running Conditions
Reduced Denaturing (10 %)
Loading amount
20 µg
Specification
HEK 293 transfected with human bestrophin 1
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk, PBS-Tween
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
non transfected HEK-293 cells lysate
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 67 kDa
Additional data
Additional Notes
Line 1. Cell lysate from HEK-293 cells transfected with human Bestrophin 1
Line 2. Cell lysate from non transfected HEK 293 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Feb 15 2008