Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pigmented skin lessions)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision Flex hight Dako
Permeabilization
No
Specification
pigmented skin lessions
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 37°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Secondary antibody
Name
Non-Abcam antibody was used: The system used for IHC is EnVision FLEX, High pH
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Mucha tinciòn y poco especìfica.
The system used for IHC is High pH (Link)
High pH is a high-sensitivity visualization system intended for use in immunohistochemistry together with Autostainer Link Instruments. The dual link system detects primary mouse and rabbit antibodies and the reaction is visualized by DAB+ Chromogen. The convenience kit includes Peroxidase-Blocking Reagent, HRP, DAB+ Chromogen, Substrate Buffer, Retrieval Solution, High pH (50x Tris/EDTA buffer, pH 9), and Wash Buffer (20x).
Abcam response
The end user has tested this antibody with mouse and IHC-P (both untested) under our Abtrial offer. A free antibody has been offered in exchange of this review.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 22 2017