Application
Western blot
Sample
Human Cell lysate - whole cell (Colon)
Loading amount
80 µg
Specification
Colon
Treatment
Basal and Knockdown (siRNA)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgG HRP secondary,
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: none? kDa Non-specific: 100, 70 and 50 kDa
Positive control
Cell-line, HT29 and HCA7 - high basal.
BCL3 knockdown samples
BCL3 knockdown samples
Negative control
SW480 and SW620 - low basal
Additional data
Additional Notes
Despite showing a weak band around 50kDa, QPCR data suggest this is not the correct band. No disappearance of a band in the knockdown samples or variation in basal levels. Try ab49470 instead
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 23 2012