Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were MeOH-fixed, immunofluorescently labelled and counterstained with DAPI. Under these conditions, this antibody produces a weak staining pattern that does not appear specific – there is no nuclear enrichment and the mitochondrial association is not readily apparent. Paraformaldehyde fixed samples do not give the expected staining pattern, rather they produce a nucleolar enriched staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Dec 08 2017