Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat T-cells)
Loading amount
25 µg
Specification
Jurkat T-cells
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BLOTTO
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit HRP
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~28 kDa Non-specific: ~35 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jonathan Rud
Verified customer
投稿 Feb 11 2010