Anti-Bcl-2 抗体 [100/D5] - BSA and Azide free (ab212800)
Key features and details
- Mouse monoclonal [100/D5] to Bcl-2 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Bcl-2 antibody [100/D5] - BSA and Azide free
Bcl-2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [100/D5] to Bcl-2 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow非交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Bcl-2 aa 1-100 (Cysteine residue).
Database link: P10415 -
ポジティブ・コントロール
- Jurkat, K562, HL60, or HeLa Cells. Tonsil or follicular lymphomas. Non- Hodgkin's lymphoma tissue. Human skin lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
ab212800 is purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
100/D5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab212800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa.
|
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Flow Cyt |
Use 1-2µg for 106 cells.
(in 0.1ml volume) |
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(for 30 minutes at RT) |
特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
Flow Cyt
Use 1-2µg for 106 cells. (in 0.1ml volume) |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (for 30 minutes at RT) |
ターゲット情報
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機能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
組織特異性
Expressed in a variety of tissues. -
関連疾患
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
翻訳後修飾
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
細胞内局在
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281020 Cow
- Entrez Gene: 596 Human
- Omim: 151430 Human
- SwissProt: O02718 Cow
- SwissProt: P10415 Human
- Unigene: 150749 Human
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別名
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
画像
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Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800) at 1 µg/ml + Human skin lysate
Predicted band size: 26 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-Hodgkin's Lymphoma tissue labeling Bcl-2 with ab212800 at 1 μg/ml.
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Flow cytometric analysis of Jurkat cells labeling Bcl-2 uwith ab212800 at 1µg/106 cells (red), compared with an isotype control (green).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab212800 は論文での使用が確認できていません。