Anti-Band 3/AE 1 (phospho Y359) 抗体 [EPR1427Y] (ab77236)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y]
Band 3/AE 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Band 3/AE 1 (phospho Y359).
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ポジティブ・コントロール
- Red blood cell lysates treated with pervanadate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1427Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77236の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000. Predicted molecular weight: 102 kDa.
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特記事項 |
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WB
1/50000. Predicted molecular weight: 102 kDa. |
ターゲット情報
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機能
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. -
組織特異性
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871). -
関連疾患
Ovalocytosis, Southeast Asian
Spherocytosis 4
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Cryohydrocytosis -
配列類似性
Belongs to the anion exchanger (TC 2.A.31) family. -
翻訳後修飾
Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated. -
細胞内局在
Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney. - Information by UniProt
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参照データベース
- Entrez Gene: 6521 Human
- Omim: 109270 Human
- SwissProt: P02730 Human
- Unigene: 210751 Human
- Unigene: 443948 Human
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別名
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
see all
画像
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All lanes : Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution
Lane 1 : Red blood cell lysate, untreated.
Lane 2 : Red blood cell lysate treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab77236 は論文での使用が確認できていません。