Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HCT116 p53-/- cell line)
Specification
HCT116 p53-/- cell line
Total protein in input
100 µg
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NET-N
Dilution
1/100
Western Blot antibody
Name
Abcam antibody:Anti-rabbit IgG VeriBlot for IP secondary antibody (HRP)
Dilution
1/2000
Additional data
Additional Notes
The IP was performed with 100 ´g WCE. Afterwards the whole precipitated sample was loaded on a 13% SDS gel. Additionally 10 ´g of the WCE was used as input.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
投稿 Aug 27 2013