Anti-Lethal factor 抗体 [5F502.2] (ab13814)
Key features and details
- Mouse monoclonal [5F502.2] to Lethal factor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Lethal factor antibody [5F502.2]
Lethal factor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5F502.2] to Lethal factor -
由来種
Mouse -
特異性
The antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Lethal factor.
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ポジティブ・コントロール
- 0.1 ug recombinant protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
5F502.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13814の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 90 kDa.
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特記事項 |
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 90 kDa. |
ターゲット情報
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関連性
The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates. -
細胞内局在
secreted -
別名
- Anthrax lethal toxin endopeptidase component antibody
- Anthrax LF antibody
- Bacillus anthracis lethal factor antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (2)
ab13814 は 2 報の論文で使用されています。
- Kim SK et al. Comparative analysis of virulence factors secreted by Bacillus anthracis Sterne at host body temperature. Lett Appl Microbiol 54:306-12 (2012). PubMed: 22268495
- Geddie ML et al. Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. J Biol Chem 280:35641-6 (2005). PubMed: 16118206