Application
Western blot
Sample
Mouse Tissue lysate - whole (white adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Treatment
WT versus KO
Specification
white adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
biorad clarity
Negative control
Bscl2 deficient mice
Exposure
5 second(s)
Bands
Specific: 48 kDa
Positive control
wild type mouse
Additional data
PubMed ID
GR73492-2: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Xavier Prieur
Verified customer
投稿 Jul 28 2014