Application
Western blot
Sample
Mouse Tissue lysate - whole (cerebellum & lower portion of brainstem (adult))
Loading amount
20 µg
Specification
cerebellum & lower portion of brainstem (adult)
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk powder in TBS Tween
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase-conjugated IgG Fraction monoclonal mous
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
SuperSignal West Dura
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ca. 43 kDa
Positive control
lower part of the brainstem containing the inferior olive
Negative control
not loaded
Additional data
Additional Notes
Lane 1 cerebellum (adult);
Lane 2 lower part of brainstem (adult).
Lane 2 lower part of brainstem (adult).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 15 2011